LIAO Wenzhu^1;2;3;4; KANG Shuhe^1;2;3;4; LI Li^1;2;3;4; YUAN Mengyao^1;2;3;4; AN Lili^1;2;3;4;

Abstract：

The process conditions for purifying crude polysaccharides from Lanzhou lily were optimized using macroporous adsorption resin, and the whitening, moisturizing, and hygroscopic activities of both crude and purified polysaccharides were evaluated. Through static experiments, DM301 was selected as the optimal resin. Based on dynamic experiments, the weighted comprehensive score (including decolorization rate, protein removal rate, and polysaccharide retention rate) was used as the response value to optimize the optimal process conditions for DM301 purification of lily polysaccharides using response surface methodology. The whitening activity was evaluated by the inhibition of Polysaccharide on monophenolase and diphenolase. The moisture retention and hygroscopic activities of the samples were investigated at 22℃ and relative humidity of 11%, 43%, 81% and 95% within 24 hours. Under the conditions of a sample concentration of 4.30 mg·mL^-1, eluent volume of 0.70 BV, flow rate of 1.36 BV·h^-1, and sample volume of 1.0 BV, the comprehensive score reaches 63.99%±0.02% (n=3), which is close to the predicted value, indicating that the established mathematical model is reasonable. The purified polysaccharides exhibited good whitening activity, which IC₅₀ values for the inhibition rates of tyrosinase monophenolase and diphenolase are 3.52 mg·mL^-1 and 2.79 mg·mL^-1, respectively. At 22℃ and four humidity conditions, both crude polysaccharides and purified polysaccharides exhibit excellent moisture absorption properties (with a 24-hour moisture absorption rate greater than 100%). Purified polysaccharides and their mixtures have high moisturizing properties (24-hour moisturizing rate greater than 97.5%). The optimization of the purification process of crude lily polysaccharides using macroporous adsorption resin combined with response surface methodology is stable and feasible, providing a theoretical basis for the effective purification of lily polysaccharides.The whitening, moisturizing, and hygroscopic activities of purified lily polysaccharides have been enhanced, providing experimental basis for the development of Lanzhou lily polysaccharides.

Key Words： Lanzhou lily polysaccharide;macroporous adsorption resin;response surface methodology;whitening activity;hygroscopicity and moisture retention

Abstract:

Keywords:

Foundation: 甘肃省教育厅高校教师创新基金(2025B-036);国家自然科学基金(21865028);2024西北民族大学化学创新团队基金(1001660139,1001660141)。

Authors: LIAO Wenzhu^1;2;3;4; KANG Shuhe^1;2;3;4; LI Li^1;2;3;4; YUAN Mengyao^1;2;3;4; AN Lili^1;2;3;4;

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